Uruguay became the final country to qualify for next year's World Cup after playing out a goalless draw with Jordan to win 5-0 on aggregate.
Cameroon will be boosted by the presence of Samuel Eto'o for their World Cup qualifying play-off against Tunisia in Rades on Sunday.
Spain won the World Cup for the first time on Sunday, with Andres Iniesta netting in extra-time to see off Holland 1-0.
Holland made it three wins from as many Group E matches with victory in their dead rubber against Cameroon in Cape Town.
Holland will aim to wrap up top spot in Group E when they meet already-eliminated Cameroon in Cape Town on Thursday.
Denmark hit back from an early deficit to re-ignite their World Cup challenge and in the process extinguish Cameroon's chances.
Group E's bottom two teams face off knowing a win is essential to keep their World Cup dreams alive.
Keisuke Honda's close-range strike near the end of the first half was enough to give Japan a 1-0 win over Cameroon.
Japan and Cameroon get their World Cup campaigns under way at Bloemfontein.
Serbia came out on top in a rollercoaster encounter with Cameroon after a 4-3 victory in Belgrade on Saturday.
Raul Meireles scored in each half to help Portugal earn a 3-1 win over 10-man Cameroon in a World Cup warmup game Tuesday.
Eyong Enoh scored late on to earn a draw for Cameroon in their friendly with fellow World Cup qualifiers Slovakia.
Cameroon's World Cup preparations took a blow on Tuesday as they were held 0-0 by Georgia.
Italy coach Marcello Lippi admits his squad is below full strength but believes the Azzurri can still test Cameroon.